Study on Two Speices of Russula by Infrared Spectroscopy Combined with Curve-fitting Analysis

Abstract

Abstract: Fourier transform infrared spectrometer was used to qualitative analysis the nutrients of Russula virescens and Russula vinosa. The infrared spectra showed that the main nutrients in these two mushroom species were polysaccharides, proteins and lipids. In the characteristic region of infrared spectra, the absorption bands were overlapped with each other, resulting in some spectral information were covered and difficult to obtain the contain information of nutrients. In this paper, second derivative spectroscopy, Fourier self-deconvolution spectroscopy combined with curve-fitting analysis were used to mine the hidden information of overlapping bands. The curve-fitting analysis of the peaks at around 1319, 1151, 936 and 887 cm^-1 showed that the content of β-D-glucan in Russula virescens was lower than that in Russula vinosa, but the content of starch was just the opposite. The results showed that Fourier transform infrared spectroscopy combined with data mining method could qualitative analysis the nutrients in mushrooms rapidly.

Key words: infrared spectroscopy, curve-fitting analysis, polysaccharides, Russula virescens, Russula vinosa

CLC Number:

O657.3

Li Dongyu, Wang Yuan, Hu Fene, Yan Cuiqiong. Study on Two Speices of Russula by Infrared Spectroscopy Combined with Curve-fitting Analysis[J]. JOURNAL OF QUJING NORMAL UNIVERSITY, 2020, 39(3): 41-46.

References

[1] 刘晓亮,图力古尔,王向华. 东北大小兴安岭地区的红菇属物种多样性[J]. 菌物学报,2017, 36(10) : 1355-1368.
[2] 朱磊. 变绿红菇多糖的分离纯化与结构分析[D]. 长春:东北师范大学,2018.
[3] LIU Y, ZHANG J J, MENG Z L. Purification, characterization and anti-tumor activities of polysaccharides extracted from wild Russula griseocarnosa[J]. International Journal of Biological Macromolecules, 2018, 109:1054-1060.
[4] KHATUA S, CHANDRA S, ACHARYA K. Expanding knowledge on Russula alatoreticula, a novel mushroom from tribal cuisine, with chemical and pharmaceutical relevance[J]. Cytotechnology, 2019,71(1): 245-259.
[5] ISAKA M, YANGCHUM A, WONGKANOUN S, et al. Marasmane and normarasumane sesquiterpenenoids from the edible mushroom Russula nigricans[J]. Phytochemistry Letters, 2017, 21: 174-178.
[6] WU J N, NIU Y W, JIAO Y C, et al. Fungal chitosan from Agaricus bisporus (Lange) Sing. Chaidam increased the stability and antioxidant activity of liposomes modified with biosurfactants and loading betulinic acid[J]. International Journal of Biological Macromolecules, 2019, 123:291-299.
[7] MA Y H, HE H Q, WU J Z, et al. Assessment of Polysaccharides from Mycelia of genus Ganoderma by Mid-Infrared and Near-Infrared Spectroscopy[J]. Scientific Reports, 2018, 8:1-10.
[8] YAO S, LI T, LI J Q, et al. Geographic identification of Boletus mushrooms by data fusion of FT-IR and UV spectroscopies combined with multivariate statistical analysis[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, 198:257-263.
[9] MEENU M, Xu B J. Application of vibrational spectroscopy for classification, authentication and quality analysis of mushroom: A concise review[J]. Food Chemistry, 2019, 289:545-557.
[10] SHARMA N, KHAJURIA Y, SHARMA J, et al. Microscopic, elemental and molecular spectroscopic investigations of root-knot nematode infested okra plant roots[J]. Vacuum, 2018, 158:126-135.
[11] CRISTINA B S, CHIOU B S, TINA W, et al. Vitamin D-fortified chitosan films from mushroom waste[J]. Carbohydrate Polymers, 2017, 167:97-104.
[12] SUN C, JIN L F, CAI Y T, et al. (1→3)-β-D-glucan from yeast cell wall: Characteristic and potential application in controlling postharvest disease of pear[J]. Postharvest Biology and Technology, 2019, 154:105-114.
[13] SRIKRAM A, SUPAPVANICH S. Proximate compositions and bioactive compounds of edible wild and cultivated mushrooms from Northeast Thailand[J]. Agriculture and Natural Resources, 2016, 50(6):432-436.
[14] ANNA V T, ANDREI V S, YULIA A D, et al. Ftir spectroscopic study of biofilms formed by the rhizobacterium Azospirillum brasilense Sp245 and its mutant Azospirillum brasilense Sp245.1610[J]. Journal of Molecular Structure, 2017, 1140:142-147.
[15] NG C H, CHEN Y, CHNG Y S, et al. Application of mid-infared spectroscopy with multivariate analysis for the discrimination of toxic plant, Gelsemium elegans[J]. Vibrational Spectroscopy, 2018, 99:13-24.
[16] DANKAR I, HADDARAH A, OMAR F E, et al. Characterization of food additive-potato starch complexes by FTIR and X-ray diffraction[J]. Food Chemistry, 2018, 260:7-12.
[17] YUAN Y, LIU Y, LIU M D, et al. Optimization extraction and bioactivities of polysaccharide from wild Russula griseocarnosa[J]. Saudi Pharmaceutical Journal, 2017, 25: 523-530.