Chloroplast Genomic Characterization and Phylogenetic Analysis of Tagetes Species

Abstract

Abstract: Tagetes is an annual herbaceous plant with important medicinal and economic value. Studying its chloroplast genome can provide a basis for the identification and interspecific genetic analysis of marigold species. Seven chloroplast genome sequences of Tagetes species were downloaded from the NCBI database, and were used to analyze their characteristics, codon preference, and phylogenetic relationships, by using bioinformatics software. The results showed that the chloroplast genome of Tagetes species exhibited typical quadripartite circular structure, ranging from 151 893 to 153 689 bp, and encoding 130 genes. The chloroplast genome was mainly composed of forward and palindrome repeats. An average of 61 SSRs was detected mostly consisting of A/T base repeats. The chloroplast genome structure was conserved. Twenty-nine high-frequency codons were detected, of which 12 ended with A and 16 ended with T, which implied a preference for codons ending with A/T. Phylogenetic analysis showed that the species of the Tagetes genus formed a monophyletic group, and the relationship between T. erecta and T. minuta was relatively closed. A relatively ancient species, Tagetes lemmonii was located at the base of the branch.

Key words: Tagetes, chloroplast genome, phylogenetics, structure characteristics

CLC Number:

LIU Chao, YE Xiuchuan, YANG Chunhui, HE Shuhui, HAN Lihong. Chloroplast Genomic Characterization and Phylogenetic Analysis of Tagetes Species[J]. Journal of Qujing Normal University, 2024, 43(3): 24-30.

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