Study On the Isolation and Identification of Secondary Metabolites of a strain of Endophytic Fungi from Veratrum nigrum Linn

JOURNAL OF QUJING NORMAL UNIVERSITY ›› 2018, Vol. 37 ›› Issue (6): 41-45.

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Study On the Isolation and Identification of Secondary Metabolites of a strain of Endophytic Fungi from Veratrum nigrum Linn

Ji Zhilin¹, Guan Huimin¹, Yang Benshou², Chen Youwei³

1.Department of Basic Medicine, Chuxiong Medical Colleage,Chuxiong Yunnan 675000, China;
2.Institute of Microbiology, Qujing Medical Colleage,Qujing Yunnan 655011, China;
3. Yunnan Institute of Microbiology, Yunnan University, Kunming Yunnan 650091, China

Received:2018-05-23 Online:2018-11-26

Abstract

Abstract: Objective To study the secondary metabolites of an endophytic fungus Ym 312628, an endophytic fungus with the activity of alkaloid, to determine the structure of the compound in order to provide a wider source of alkaloid drugs. Methods The optimum fermentation conditions of strain Ym 312628 were determined by orthogonal experiment, and the fermentation products were purified and separated by various chromatographic columns, and the chemical structure was determined by spectral analysis. Results 7 compounds were isolated and 3 of them were identified as 3- phenyl lactic acid, 5- butyl pyridine formic acid, and beer sterol. The specific structure of 1 ergosterols was further identified, and three alkaloid positive reactions were identified as a class of structural analogues with nucleotide units, but the specific chemical junctions were identified. Further identification is required. Conclusion The metabolite of strain Ym 312628 3- phenyl lactic acid provides an effective way to produce Salviae Miltiorrhizae substitutes.

Key words: Veratrum nigrum Linn, Endophytic fungi, Secondary Metabolites

CLC Number:

Q936

Ji Zhilin, Guan Huimin, Yang Benshou, Chen Youwei. Study On the Isolation and Identification of Secondary Metabolites of a strain of Endophytic Fungi from Veratrum nigrum Linn[J]. JOURNAL OF QUJING NORMAL UNIVERSITY, 2018, 37(6): 41-45.

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Study On the Isolation and Identification of Secondary Metabolites of a strain of Endophytic Fungi from Veratrum nigrum Linn

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