飞龙斩血内一株链霉菌属的分离鉴定及抑菌活性检测

曲靖师范学院学报 ›› 2017, Vol. 36 ›› Issue (3): 30-34.

飞龙斩血内一株链霉菌属的分离鉴定及抑菌活性检测

杨本寿^{1, 2}, 杨小珍¹, 杨德^{3, *}

1. 曲靖医学高等专科学校微生物研究所,云南曲靖 655000;
2.曲靖师范学院,云南曲靖 655000;
3.云南农业大学园林园艺学院,云南昆明 650091

收稿日期:2017-03-15 出版日期:2017-05-26
通讯作者: 杨德,云南农业大学园林园艺学院教授,博士生导师,主要从事植物资源评价与利用研究.
作者简介:杨本寿,曲靖医学高等专科学校微生物研究所副教授,博士研究生,主要从事植物资源评价与利用研究.
基金资助:
云南省高校“云南特境内生菌资源的开发与利用科技创新团队”

Isolation, Identification and Bioactivity Screening of a Streptomyces Strain in Toddalia Asiatica L.

Yang Benshou^{1, 2}, YangXiaozhen¹, Yang De³

1. Institute of Microbiology, Qujing Medical College, Qujing Yunnan, 655000;
2. Qujing Normal University, Qujing Yunnan, 655000;
3. Landscape Gardening Institute, Yunnan Agricultural University , Kunming Yunnan, 650091,China

Received:2017-03-15 Published:2017-05-26

摘要/Abstract

摘要： 采用严格的表面消毒程序以及在培养基中添加抑菌剂的方法从中药植物飞龙斩血中分离得到一株内生放线菌F-3-003, 菌株的发酵产物通过纸片扩散法体外检测具有一定的抑菌活性.菌落形态特征和23S rDNA序列分析表明,菌株F-3-003属于链霉菌属,通过16S rDNA序列分析显示菌株F-3-003与多株淡紫灰链霉菌(Streptomyces lavendulae)的序列同源性均高于99 %,因此,将菌株F-3-003命名为淡紫灰链霉菌F-3-003,基因登录号为KF751667,本研究为飞龙斩血内生菌资源的开发利用提供了基础.

关键词: 飞龙斩血, 分类鉴定, 抑菌活性, 淡紫灰链霉菌

Abstract: An endophytic actinomycetes strain F-3-003 was obtained from T. asiatica. by the conventional method. It’s detected that its fermentation broth had some antibacterial activity against gram-positive bacteria by disc diffusion method. The strain F-3-003 was classified and identified as streptomyces sp. based on its morphological characters and ribosomal 23S rDNA sequence analysis. The 16S rDNA sequence analysis homology between strain F-3-003 and many other strains of streptomyces lavendulae was higher than 99%. So, the strain F-3-003 was named as streptomyces lavendulae F-3-003 while its gene accession number was numbered as KF751667. This research provides a basis for utilizing endophytic resourcesfrom T. asiatica.

Key words: toddalia asiatica, identification, antibacterial activity, streptomyces lavendulae

中图分类号:

Q93-331S567.3

杨本寿, 杨小珍, 杨德. 飞龙斩血内一株链霉菌属的分离鉴定及抑菌活性检测[J]. 曲靖师范学院学报, 2017, 36(3): 30-34.

Yang Benshou, YangXiaozhen, Yang De. Isolation, Identification and Bioactivity Screening of a Streptomyces Strain in Toddalia Asiatica L.[J]. JOURNAL OF QUJING NORMAL UNIVERSITY, 2017, 36(3): 30-34.