JOURNAL OF QUJING NORMAL UNIVERSITY ›› 2017, Vol. 36 ›› Issue (3): 30-34.

• Orginal Article • Previous Articles     Next Articles

Isolation, Identification and Bioactivity Screening of a Streptomyces Strain in Toddalia Asiatica L.

Yang Benshou1, 2, YangXiaozhen1, Yang De3   

  1. 1. Institute of Microbiology, Qujing Medical College, Qujing Yunnan, 655000;
    2. Qujing Normal University, Qujing Yunnan, 655000;
    3. Landscape Gardening Institute, Yunnan Agricultural University , Kunming Yunnan, 650091,China
  • Received:2017-03-15 Online:2017-05-26

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Abstract: An endophytic actinomycetes strain F-3-003 was obtained from T. asiatica. by the conventional method. It’s detected that its fermentation broth had some antibacterial activity against gram-positive bacteria by disc diffusion method. The strain F-3-003 was classified and identified as streptomyces sp. based on its morphological characters and ribosomal 23S rDNA sequence analysis. The 16S rDNA sequence analysis homology between strain F-3-003 and many other strains of streptomyces lavendulae was higher than 99%. So, the strain F-3-003 was named as streptomyces lavendulae F-3-003 while its gene accession number was numbered as KF751667. This research provides a basis for utilizing endophytic resourcesfrom T. asiatica.

Key words: toddalia asiatica, identification, antibacterial activity, streptomyces lavendulae

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