万寿菊属物种叶绿体基因组特征及系统发育分析

曲靖师范学院学报 ›› 2024, Vol. 43 ›› Issue (3): 24-30.

万寿菊属物种叶绿体基因组特征及系统发育分析

刘潮, 叶秀传, 杨春会, 何淑慧, 韩利红

曲靖师范学院生物资源与食品工程学院;云南省高校特色果酒技术创新与应用工程研究中心,云南曲靖 655011

收稿日期:2023-11-13 出版日期:2024-05-26 发布日期:2024-06-14
通讯作者: 韩利红,曲靖师范学院生物资源与食品工程学院教授,博士,主要从事真菌系统发育与进化研究.
作者简介:刘潮,曲靖师范学院生物资源与食品工程学院副教授,博士,主要从事植物系统发育与进化研究.
基金资助:
国家自然科学基金“丛枝菌根真菌(AMF)促进辣椒磷吸收相关microRNA的鉴定及功能研究”(32060710).

Chloroplast Genomic Characterization and Phylogenetic Analysis of Tagetes Species

LIU Chao, YE Xiuchuan, YANG Chunhui, HE Shuhui, HAN Lihong

School of Biological Resource and Food Engineering, Yunnan Engineering Research Center of Fruit Wine,Qujing Normal University, Qujing Yunnan 655011,China

Received:2023-11-13 Published:2024-05-26 Online:2024-06-14

摘要/Abstract

摘要： 万寿菊属(Tagetes)为菊科一年生草本植物,具有重要的药用和经济价值,对其开展叶绿体基因组研究,可为该属物种的种质资源鉴定及种间遗传分析提供参考. 从NCBI数据库下载万寿菊属物种的7条叶绿体基因组序列,借助生物信息学软件对叶绿体基因组的特征、密码子使用性及系统发育进行分析. 结果表明,万寿菊属物种叶绿体基因组具有典型的四分体结构,叶绿体基因组介于151 893～153 689 bp,编码130个基因. 叶绿体基因组以正向重复和回文重复为主,SSR位点数平均61个,多为A/T碱基重复. 叶绿体基因组结构保守,高频密码子有29个,以A或T结尾的分别有12个和16个,偏好使用A/T结尾的密码子. 系统发育分析表明,万寿菊属物种构成单系群,万寿菊与印加孔雀草亲缘关系较近,芳香万寿菊位于分支基部,属于较古老的物种.

关键词: 万寿菊属, 叶绿体基因组, 系统发育, 结构特征

Abstract: Tagetes is an annual herbaceous plant with important medicinal and economic value. Studying its chloroplast genome can provide a basis for the identification and interspecific genetic analysis of marigold species. Seven chloroplast genome sequences of Tagetes species were downloaded from the NCBI database, and were used to analyze their characteristics, codon preference, and phylogenetic relationships, by using bioinformatics software. The results showed that the chloroplast genome of Tagetes species exhibited typical quadripartite circular structure, ranging from 151 893 to 153 689 bp, and encoding 130 genes. The chloroplast genome was mainly composed of forward and palindrome repeats. An average of 61 SSRs was detected mostly consisting of A/T base repeats. The chloroplast genome structure was conserved. Twenty-nine high-frequency codons were detected, of which 12 ended with A and 16 ended with T, which implied a preference for codons ending with A/T. Phylogenetic analysis showed that the species of the Tagetes genus formed a monophyletic group, and the relationship between T. erecta and T. minuta was relatively closed. A relatively ancient species, Tagetes lemmonii was located at the base of the branch.

Key words: Tagetes, chloroplast genome, phylogenetics, structure characteristics

中图分类号:

刘潮, 叶秀传, 杨春会, 何淑慧, 韩利红. 万寿菊属物种叶绿体基因组特征及系统发育分析[J]. 曲靖师范学院学报, 2024, 43(3): 24-30.

LIU Chao, YE Xiuchuan, YANG Chunhui, HE Shuhui, HAN Lihong. Chloroplast Genomic Characterization and Phylogenetic Analysis of Tagetes Species[J]. Journal of Qujing Normal University, 2024, 43(3): 24-30.

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