曲靖师范学院学报 ›› 2022, Vol. 41 ›› Issue (3): 16-28.

• 生物多样性研究 • 上一篇    下一篇

云南竹生子囊菌的物种鉴定与多样性调查

代冬琴, 韩莉苏, 金星辰   

  1. 曲靖师范学院 生物资源与食品工程学院,云南 曲靖 655011
  • 收稿日期:2022-04-25 出版日期:2022-05-26 发布日期:2022-06-02
  • 作者简介:代冬琴,曲靖师范学院生物资源与食品工程学院副教授,主要从事真菌分类、分子系统与多样性研究.
  • 基金资助:
    国家自然科学基金“云南竹生子囊菌的多样性、分类及系统发育研究”(NSFC 31760013);云南省高层次人才引进“青年人才专项”项目“西南竹生子囊菌多样性、系统学及代谢产物应用研究”.

Species Identification and Diversity Investigation of Bambusicolous Ascomycetesin in Yunnan

DAI Dongqin, HAN Lisu, JIN Xingchen   

  1. School of Biological Resources and Food Engineering, Qujing Normal University, Qujing Yunnan 655011, China
  • Received:2022-04-25 Published:2022-05-26 Online:2022-06-02

摘要: 云南竹林分布广泛,竹子上寄生的真菌种类繁多,其中大部分是子囊菌.通过对云南滇中、滇南、滇东北、滇西北地区竹子上寄生和腐生的子囊菌进行野外采集、室内分离、DNA提取、测序和鉴定分析,探究云南竹生子囊类群的物种多样性与分布情况.研究样本共300份,通过单孢分离或组织分离方法成功获得纯培养菌株,采用分子方法,鉴定物种74种,统计了云南竹生子囊菌在各个区域的分布数据,说明滇南竹区子囊菌物种多样性较为丰富,滇西北地处高海拔区域,其物种多样性较为偏低.项目进行了竹生子囊菌各纲、目、科、属的多样性分析,数据表明云南竹生子囊菌类群主要隶属座囊菌纲(Dothideomycetes)和粪壳菌纲(Sordariomycetes),分布较多的目为Botryosphaeriales、Hypocreales、Pleosporales和Xylariales,分布较多的科为Apiosporaceae、Bambusicolaceae、Botryosphaeriaceae、Roussoellaceae和Xylariaceae,分布较多的属为Arthrinium、Bambusicola和Roussoella, Arthrinium和Botryosphaeriaceae是植物常见病原菌类群,该发现对植物的病害防控与资源保护具有参考价值.

关键词: 子囊菌, 物种多样性, 座囊菌纲, 粪壳菌纲

Abstract: Bamboo forests are widely distributed in Yunnan. Numerous fungi inhabit on bamboo host, most of which are ascomycetes. In this study, the species diversity and distributions of bambusicolous ascomycetes were investigated based on the data collected from central, south, northeast and northwest of Yunnan. Fungal isolation, DNA extraction, sequencing and identification were carried out. Totally, 300 samples were collected, and pure cultures were obtained by using methods of single spore isolation and tissue isolation. Seventy-four species were identified based on molecular analyses. Bamboo ascomycetes distributions of each area of Yunnan were statistically recorded. The research shows that fungal species diversity in the southern area is relatively rich, however, in the northwest of Yunnan, it demonstrates lower due to the high altitude. The species diversities in classes, orders, families and genera were carried out to show that species mainly belong to classes Dothideomycetes, Sordariomycetes and orders Botryosphaeriales, Hypocreales, Pleosporales and Xylariales. The most widely distributed families are Apiosporaceae, Bambusicolaceae, Botryosphaeriaceae, Roussoellaceae and Xylariaceae. Arthrinium, Bambusicola and Roussoella were the most widely distributed genera. Members of Arthrinium and Botryosphaeriaceae are common plant pathogens. The discovery has reference value for plant disease control and resource conservation.

Key words: Ascomycetes, species diversity, Dothideomycetes, Sordariomycetes

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