一株藜芦植物内生真菌代谢产物的分离鉴定研究

曲靖师范学院学报 ›› 2018, Vol. 37 ›› Issue (6): 41-45.

一株藜芦植物内生真菌代谢产物的分离鉴定研究

姬志林¹,关会敏¹,杨本寿²,陈有为³

1.楚雄医药高等专科学校基础医学系,云南楚雄 675000;
2.曲靖医学高等专科学校微生物研究所,云南曲靖 655000;
3.云南大学云南省微生物研究所,云南昆明 650091

收稿日期:2018-05-23 出版日期:2018-11-26
通讯作者: 陈有为,云南大学云南省微生物研究所副研究员,主要从事微生物真菌资源学和应用微生物学研究.
作者简介:姬志林,楚雄医药高等专科学校讲师,主要从事微生物学和基础医学研究.

Study On the Isolation and Identification of Secondary Metabolites of a strain of Endophytic Fungi from Veratrum nigrum Linn

Ji Zhilin¹, Guan Huimin¹, Yang Benshou², Chen Youwei³

1.Department of Basic Medicine, Chuxiong Medical Colleage,Chuxiong Yunnan 675000, China;
2.Institute of Microbiology, Qujing Medical Colleage,Qujing Yunnan 655011, China;
3. Yunnan Institute of Microbiology, Yunnan University, Kunming Yunnan 650091, China

Received:2018-05-23 Published:2018-11-26

摘要/Abstract

摘要： 目的:对分离到的一株具产生物碱活性的藜芦植物内生真菌Ym 312628进行次生代谢产物研究,确定其化合物结构,以期为生物碱类药物提供更广阔的来源.方法:应用正交实验,跟踪生物碱活性,确定菌株Ym 312628的最佳发酵条件,应用各种层析柱对其发酵产物进行纯化分离,经光谱分析确定其化学结构.结果:分离得到7个化合物,其中3个结构鉴定为3-苯基乳酸、5-丁基吡啶甲酸、啤酒甾醇,1个麦角甾醇类物质具体结构还需进一步鉴定;以及三个生物碱阳性反应物质,初步鉴定为一类带有核苷酸单元的结构类似物,但具体化学结构需进一步鉴定.结论:菌株Ym 312628的代谢产物3-苯基乳酸对生产丹参素的替代品提供了有效途径.

关键词: 藜芦植物, 内生真菌, 次生代谢产物

Abstract: Objective To study the secondary metabolites of an endophytic fungus Ym 312628, an endophytic fungus with the activity of alkaloid, to determine the structure of the compound in order to provide a wider source of alkaloid drugs. Methods The optimum fermentation conditions of strain Ym 312628 were determined by orthogonal experiment, and the fermentation products were purified and separated by various chromatographic columns, and the chemical structure was determined by spectral analysis. Results 7 compounds were isolated and 3 of them were identified as 3- phenyl lactic acid, 5- butyl pyridine formic acid, and beer sterol. The specific structure of 1 ergosterols was further identified, and three alkaloid positive reactions were identified as a class of structural analogues with nucleotide units, but the specific chemical junctions were identified. Further identification is required. Conclusion The metabolite of strain Ym 312628 3- phenyl lactic acid provides an effective way to produce Salviae Miltiorrhizae substitutes.

Key words: Veratrum nigrum Linn, Endophytic fungi, Secondary Metabolites

中图分类号:

Q936

姬志林,关会敏,杨本寿,陈有为. 一株藜芦植物内生真菌代谢产物的分离鉴定研究[J]. 曲靖师范学院学报, 2018, 37(6): 41-45.

Ji Zhilin, Guan Huimin, Yang Benshou, Chen Youwei. Study On the Isolation and Identification of Secondary Metabolites of a strain of Endophytic Fungi from Veratrum nigrum Linn[J]. JOURNAL OF QUJING NORMAL UNIVERSITY, 2018, 37(6): 41-45.

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