滇龙胆内生真菌的分离及其抑菌活性研究

曲靖师范学院学报 ›› 2017, Vol. 36 ›› Issue (6): 33-36.

滇龙胆内生真菌的分离及其抑菌活性研究

宋艳秋¹,刘卫红²

1.大理大学公共卫生学院;
2.大理大学图书馆,云南大理 671000

收稿日期:2017-10-20 出版日期:2017-12-10
作者简介:宋艳秋,大理大学公共卫生学院讲师,主要从事微生物学研究.
基金资助:
国家自然科学基金“内生菌对滇龙胆道地性的影响及其机制初步研究”(81102806);云南省基础应用基金“内生菌对滇龙胆品质的影响及其机制初步研究”(2011FZ166)

Isolation and Antimicrobial Activity of Endophytic Fungi from Yunnan Gentiana

Song Yanqiu¹,Liu Weihong²

1. College of Public Health, Dali University;
2. Library, Dali University, Dali Yunnan, 671000, China

Received:2017-10-20 Published:2017-12-10

摘要/Abstract

摘要： 为了解云南道地药材滇龙胆内生真菌的菌落结构及代谢产物的抑菌活性,采用组织块法分离健康滇龙胆植株根、茎、叶中的内生真菌,并根据菌落和镜下特征对其进行初步形态学鉴定.并通过滤纸片法对分离得到的内生真菌进行抑菌活性筛选.从滇龙胆根、茎、叶中共分离到49株内生真菌,分别属于15个属,其中有11属,23株对至少1种指示菌有抗菌活性,占总分离菌株的46.9%.本试验研究了滇龙胆内生真菌的种群分布,获得了一些具有抑菌活性的内生真菌,为寻找新的抗菌药物奠定基础.

关键词: 滇龙胆, 内生真菌, 抑菌活性

Abstract: In order to understand the colony structure and antimicrobial activity of endophytic fungi from Gentiana in Yunnan, the endophytic fungi in root, stem and leaf of healthy Gentiana were isolated by tissue block method, their morphological identification was carried out according to colony and microscopic characteristics, and the antifungal activity of the isolated endophytic fungi was screened by filter paper method. 49 endophytic fungi were isolated from the roots, stems and leaves of Gentiana, belonging to 15 genera, of which 11 genera and 23 strains had antibacterial activity to at least 1 indicative bacteria, accounting for 46.9% of the total Yunnan Gentiana. In this study, the population distribution of endophytic fungi in Yunnan Gentiana was studied, and some endophytic fungi with antimicrobial activity were obtained, which laid a foundation for the search for new antibacterial agents.

Key words: Yunnan Gentiana, endophytic fungi, antimicrobial activity

中图分类号:

宋艳秋,刘卫红. 滇龙胆内生真菌的分离及其抑菌活性研究[J]. 曲靖师范学院学报, 2017, 36(6): 33-36.

Song Yanqiu,Liu Weihong. Isolation and Antimicrobial Activity of Endophytic Fungi from Yunnan Gentiana[J]. JOURNAL OF QUJING NORMAL UNIVERSITY, 2017, 36(6): 33-36.

参考文献

[1] Stone J K, Bacon C W, White J. An overview of endophytic microbes: endophytism defined [J]. Microbial endophytes, 2000,3: 29-33.
[2] 陈美兰, 黄璐琦, 欧阳少华,等. 植物内生菌对道地药材形成的影响[J]. 中国中医药信息杂志, 2006, 13(9): 40-42.
[3] Gimenez C, Cabrera R, Reina M, et al. Fungal endophytes and their role in plant protection [J]. Current Organic Chemistry, 2007, 11( 8) : 707-720.
[4] Kharwar R N, Mishra A, Gond S K, et al. Anticancer compounds derived from fungal endophytes: their importance and future challenges [J]. Natural Product Reports, 2011, 28(7) : 1208-1228.
[5] 张勇.龙胆苦甙药理研究进展[J].云南医药, 1991,12(5):304-305.
[6] 苗翠苹, 余莹, 陈有为,等. 滇牡丹内生真菌的分离及其抑菌活性研究[J]. 中国药学杂志, 2011,46(10):738-741.
[7] 魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979 : 405-650.
[8] H.L巴尼特, B.B亨特.半知菌属图解[M]. 沈崇尧,译.北京: 科学出版社, 1977: 41-197.
[9] 邵士成, 吴少华,陈有为,等. 云南元江印楝植物内生真菌的种类组成[J]. 生物多样性, 2008, 16(1): 63-67.
[10] 贾敏, 游飞, 秦路平. 内生菌药用新资源与道地药材的关系探讨[J]. 药学服务与研究, 2009,9(5): 321-324.
[11] Schulz B , Boyle C. The endophytic continuum[J]. Mycological Research, 2005, 109(6): 661-686.
[12] Gao W W,Guo S X. Effects of Endophytic Fungal Hyphae and Their Metabolites on the Growth of Dendrobium candidum and Anoectochilus roxburghii[J]. Acta Academiae Medicinae Sinicae, 2001,23 (6) : 556-559.
[13] 李智敏, 赵磊, 白艳婷,等. 不同产地滇龙胆中龙胆苦苷的含量测定[J]. 云南中医学院学报, 2008, 31(6): 10-11.
[14] 赵磊, 李智敏, 杨俊,等. 不同产地滇龙胆地上部分龙胆苦苷的含量测定[J]. 云南中医学院学报, 2008, 31(5):23-25.