魔芋种间分子鉴定的SSR标记开发

曲靖师范学院学报 ›› 2022, Vol. 41 ›› Issue (3): 46-52.

魔芋种间分子鉴定的SSR标记开发

高永

曲靖师范学院生物资源与食品工程学院,云南曲靖 655011

收稿日期:2022-04-25 出版日期:2022-05-26 发布日期:2022-06-02
作者简介:高永,曲靖师范学院生物资源与食品工程学院副教授,主要从事植物资源的遗传评价与利用研究.
基金资助:
云南省地方本科高校(部分)基础研究联合专项青年项目“喀斯特攸乐魔芋的遗传多样性及谱系地理研究”(202101BA070001-011).

Development of SSR Markers for Molecular Identification of Amorphophallus Species

GAO Yong

School of Biological Resources and Food Engineering, Qujing Normal University, Qujing Yunnan 655011, China

Received:2022-04-25 Published:2022-05-26 Online:2022-06-02

摘要/Abstract

摘要： 魔芋属(Amorphophallus)属于天南星科多年生草本植物,因其富含葡甘露聚糖,在中国、日本等亚洲国家被作为经济作物种植.虽然我国栽培魔芋的历史悠久,但目前尚未有一种可简单鉴定属内各物种的方法,这对魔芋的种植产业发展造成了一定困难.简单重复序列(simple sequence repeat, SSR)被公认为作物品种及物种鉴定研究中强大、信息含量丰富的分子标记之一.通过滇魔芋的简化基因组测序数据开发了魔芋种间通用的SSR标记,并基于12个SSR标记对4个魔芋种进行了初步鉴定.结果表明,12个SSR标记的等位基因数(Na)在2～4之间,这批SSR标记可以有效地将滇魔芋和东亚魔芋鉴定出来,但对西盟魔芋和白魔芋的区分度不足.本研究为今后魔芋资源的分子鉴定提供启示.

关键词: 魔芋, SSR标记, 分子鉴定

Abstract: Genus Amorphophallus are perennial herbs that belong to the Araceae family. For the high glucomannan content in the underground corm, several species in this genus have been planted in many Asian countries, such as China and Japan. Although there is a long history of cultivation in China, the molecular identification method of Amorphophallus species is still not available, which hinders the plantation industry. Simple sequence repeat (SSR) has been considered as one of the powerful and informative molecular markers in identifying species and crop varieties. In this study, SSR markers of Amorphophallus species were developed by using restriction site-associated DNA sequencing, and 12 screened polymorphic markers were used for identifying four Amorphophallus species. The results showed that the allele number of 12 SSR markers ranged from two to four. These SSRs could distinguish A. yunnanensis and A. tonkinensis from the rest two species. This study gives hints to the molecular identification of Amorphophallus resources in the future.

Key words: Amorphophallus, SSR markers, molecular identification

中图分类号:

高永. 魔芋种间分子鉴定的SSR标记开发[J]. 曲靖师范学院学报, 2022, 41(3): 46-52.

GAO Yong. Development of SSR Markers for Molecular Identification of Amorphophallus Species[J]. Journal of Qujing Normal University, 2022, 41(3): 46-52.

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